开题报告内容：
Experiment Purposes
1.Investigate the effect of PPAR delta modulators on cancer cell viability.
2.Understand the potential role of PPAR delta in carcinogenesis.
Experiment Contents and Methods
Recently, anti-cancer drug development has been aimed to exploit cancer cell metabolism. Cancer cell lines can be classified based on the expression level of peroxisome proliferator-activated receptors (PPARs), nuclear receptors important in the regulation of cellular metabolism. More recent literature has suggested that PPAR delta antagonism may be helpful in curbing the proliferation of certain types of cancer.
In this study, the effect of PPAR delta modulators on cancer cell viability will be investigated to understand the potential role of PPAR delta in carcinogenesis. Colon cancer cell lines (HCC-2998, KM-12) are treated with anti-cancer drugs, doxorubicin and 5-fluorouracil (5-FU), and different concentration of PPAR delta agonist GW501516 or PPAR delta antagonist GSK0660 for 72hrs to identify the function of PPAR delta modulators on cancer cell viability. The cytotoxicity of anti-cancer drugs, doxorubicin and 5-fluorouracil (5-FU), will be compared between PPAR delta-positive and PPAR delta-negative colon cancer cell lines in the presence of a PPAR delta agonist GW501516 or a PPAR delta antagonist GSK0660. Cell viability will be determined using a cell counting kit-8.
Review
Abstract
Recently, anti-cancer drug development has been aimed to exploit cancer cell metabolism. Cancer cell lines can be classified based on the expression level of peroxisome proliferator-activated receptors (PPARs), nuclear receptors important in the regulation of cellular metabolism. Three PPAR isotypes have been described, alpha, gamma and delta. Roles of PPAR alpha and PPAR gamma are now quite well-known. However, functions of PPAR delta are uncompletely known to date, but more recent literature has suggested that PPAR delta antagonism may be helpful in curbing the proliferation of certain types of cancer.
