Research of pharmacology effects of herbal formulas Homalomena Occulta on white mice (Mus musculus albino var)In older cartilage cells no longer capable of reproduction and regeneration, cartilage cells and also gradually decreased synthesis function to create collagen fibers and cartilage mucopolysacarit make quality fades, especially the elasticity bearing. So arthritis, degenerative joint disease is common in older people, occurs in both women and men.In modern society , people have to work too hard not to relax more , less physically active . Disease osteoarthritis pain gradually rejuvenate most evident in the work office and porters heavy labor . The disease often causes discomfort , fatigue, prolonged pain . The patient may have to sit there , unable to perform daily activities and other diseases arise . Patients less osteoarthritis pain affects quality of life and efficiency of work and caused economic losses for patients and society . The misuse of pain medications , especially corticosteroids preparations , making the patient may have a lot of complications ( gastric lesions , duodenum , hypertension , diabetes , osteoporosis , infections , adrenal insufficiency ... ) . According to current trends , the humans have limited use of the drug group and replace it with the herb derived from nature . In folk people often use the Homalomena Occulta.Homalomena occulta (Lour.) Schott:Homalomena occulta (Lour.) Schott is herbaceous to . Long horizontal rhizomes grow beef , straight or crooked , more fuel , have broken sclerosis and fragrant. Leaves concentrated at the top of the rhizome can be up to 30 cm long , 18 cm wide . Long petioles 27-50 cm , spreading root and stem bulge occupies third stem from the bottom up .Inflorescence is a light green cotton model , each clump is usually 3-4 mo cotton . Cotton stalks long mo 5-15 cm . Part oval bearing female flowers only by a half- length bearing male flowers have no perianth . Caleb had a delicate flower shapes , with the first long stigmas , elected ovoid , translucent dots point , many ovules . The male flowers have four delicate leaves , broad filaments very short , anthers parallel . Berries contain so many particles . Blossoms in April-June , seasonal fruit from August to October .Plants have similar effects : species Homalomena tonkinensin Engl . , Homalomena gigantea Engl . , Homalomena pieereana Engl .Chemical Composition:In the rhizome has about 0.8 to 1% oil per dry roots masterpiece. Pale yellow oils, pleasant fragrance. In approximately 40% oil l-linalol, a little Terpineol and approximately 2% per linalyl acetate esters. There are also sabinen, limonen, terpinen acetaldehyde, propionic aldehydes. Research domestic and abroad:-Domestics research: Homalomena occulta (Lour.)is one of the components of the preparations for Cot Thoai Vuong, the research group Medical University Hanoi conduct research topic: 'Assessing the effects of degeneration United Essence products in therapeutic support patients with sciatica caused 'by the degeneration of the spine. Results showed patients quickly get rid of the pain, anti-inflammatory, enhance blood circulation, nourishing the vertebral disc.-Abroad research:Wang YF et al (2007 ) have isolated three new quality eudesmane sesquiterpenoid group , and 8 substances including mucrolidin knew , 1beta; , 4beta; , 7alpha; - trihydroxyeudesmane , 1beta; , 4beta; , 6beta; ,11 - terahydroxyeudesman , oplodiol , bullatantriol , acetylbullatantriol , homalomenol , maristeminol from Homalomena Occulta . These compounds have antibacterial activity of 6 different strains and most have weak antibacterial properties [ 1] .In 2008, the group of pharmaceutical research universities in China have identified two more of sesquiterpenoid ( 6.7 ) in the roots of Homalomena Occulta along with 5 previously known oplodiol , oplopanone , homalomenol C , bullatantriol , and 1beta; , 4beta; , 7alpha; - trihydroxyeudesmane , and it also proves that the quality of sesquiterpenoid group capable proliferation and differentiation of osteoblasts in vitro [ 2 ] . In 2009, the team also discovered a new substance in group 3 sesquiterpenoid is 6alpha; , 7alpha; , 10alpha; - trihydroxyisoducane have been isolated from the roots Homalomena Occulta [ 3 ] .The research content:Research of pharmacology effects of herbal formulas Homalomena Occulta on white mice The research methodology pharmacology effectsSurvey of oral acute toxicity [4], [5], [6]Studies of the acute toxicity test samples on laboratory animals, mainly determining if the average lethal dose (the dose that killed 50% of experimental animals LD50 = lethal dose 50%) in certain conditions. Know the new LD50 determined therapeutic index TI is a very important parameter to determine the therapeutic dose, usually about 1/10 of LD50 or less. Therapeutic index TI (therapeutic index) is the ratio of the average lethal dose LD50 and average effective dose ED50:TI = Conditions: TI ge; 10Survey chronic toxicity[4], [7], [8], [9]Plaster testing in distilled water and white mice drinking with volume of 0.1 ml / kg body weight for 60 consecutive days rats. Implementation of parallel batch control (distilled water only). Choose mice weighing 25 2 g. Divide treatments for rats drinking distilled water, drinking lots of testing for high mouse event coordination Homalomena Occulta . Mice drinking continuously for 2 months. Get in rat tail blood tests (blood drawn in the morning before eating rats).Reference:[1] Wang YF, Wang XY, Lai GF, Lu CH, Luo SD (2007), Tree new sesquiterpenoids from aerial parts of Homalomena occulta, Chem Biodivers, 4(5):925-931.[2] Hu YM, Liu C, Cheng KW, Sung HH, Williamsm LD, Yang ZL, Ye WC (2008) Sesquiterpenoids from Homalomena occlulta affect osteoblast proliferation, differentiation and mineralization in vitro, Phytochemistry, 69(12):2367-2373.[3] Hu YM, Yang ZL, Wang H, Ye WC (2009) Nat Prod Res, 23(14):1279-83[4] National Institute of Medicinal Materials (2006 ) , Research Methods pharmacological effects of herbal medicines , Publishing House of Science and Engineering, pages 57-63 , 140-141 , 279-284 , 295.311 to 320 .[ 5 ] Do Trung Dam (1996 ) , Methods for determination of acute toxicity of drugs , Medical Publishing House , Ha Noi , pp. 7-22 .[6] Department of Immunology - Pathophysiology Medical University of Ho Chi Minh City (2006 ) , Immunology - Pathophysiology , Medical Publishing House branch in Ho Chi Minh City .[7] Nguyen Dinh Giau , Nguyen Chi Mai , Vietnam Tran Thi Hong (2000 ) , Physiology of humans and animals , Publisher National University of Ho Chi Minh City , pp. 7-91 , 187-188 , 235 - 237 .[8] Nguyen Tien Thang , Nguyen Dinh Huyen (1998 ) , modern biochemistry curriculum , Educational Publishers , pp. 255-261 .[9] Ministry of Health , Regulation evaluate the safety and efficacy of traditional medicine , 371/BYT Decision - Decision , 12-3-1996 .
资料编号：[389672]
